Proliferum LSR is a serum-free 1:1 FBS replacement suitable for primary and immortalized cell proliferation. Multus Biotechnology has optimized its performance to work in a variety of environments including adherent culture, suspension culture, cell isolation, cryopreservation and recovery while maintaining cell proliferation and functionality over multiple passages. Fetal Bovine Serum (FBS) is often used in cell culture but is unethically sourced and has large price and performance fluctuations. Serum-free formulations are typically expensive, do not always perform well across the different cell lines, and are not designed for scale. Proliferum LSR serum substitute is designed to grow different species' mammalian myoblasts, fibroblasts and adipocytes. It has the benefits of being serum-free, adaptation-free and demonstrates high performance and versatility across cell types and scaffolding materials over multiple passages.